This study examines awareness of and experiences with pre-exposure prophylaxis (PrEP) among Black women in New York City. Black women are disproportionately impacted by HIV. In 2015, nearly two-thirds (61%) of women diagnosed with HIV were Black, despite comprising just 12% of women in the US. Biomedical HIV prevention strategies, such as PrEP represent new opportunities to reduce HIV rates among Black women, yet PrEP uptake has been low. In an effort to close this gap, this study aims to understand Black women’s awareness of PrEP, attitudes about administration and uptake, barriers to and facilitators of use, and perceived effective dissemination strategies. Such data will serve as the foundation for future research that will develop and test an intervention to increase PrEP uptake among Black women, with an emphasis toward Black women in urban environments.